NASC Stock Detail Page
NASC ID: N119
Name: 4H Proteolysis
ABRC stock number: CS119
Donation Date: 1991-06-01
| Donated by: | Max-Planck-Institute for Plant Breeding Research Andreas Bachmair |
Donor Number: 4H prt
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-8
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
There is no obvious phenotype attached to the prt1 mutation. It can be assessed by PCR: primers CAG AGG AAG AGC AAG AAC GAG AAT and CCA CCT TCT GTT TAT CTA CAC give a band which can be digested with MnlI (recognition sequence CCTC) in the wild type, but not in the mutant (change to CCTT). Line 4H prt1 carries the prt1-1 mutation in the 4H background, i.e. with transgene. The transgene confers Hygromycin resistance, but in addition, as a consequence of the homozygous presence of the prt1 mutation, it confers resistance to methotrexate (0.1mg/l).
References
Potuschak, T. et al. 1998. PRT1 of Arabidopsis thaliana encodes a component of the plant N-end rule pathway. Proceedings of the National Academy of Sciences of the United States of America 95(14):7904-8.PubMed ID: 9653113.